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Leukocyte trafficking in experimental autoimmune uveitis in vivo
Author(s) -
ParnabyPrice Adrian,
Stanford Miles R.,
Biggerstaff John,
Howe Lucy,
Whiston Roy A.,
Marshall John,
Wallace Graham R.
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.64.4.434
Subject(s) - biology , in vivo , uveitis , immunology , autoimmune disease , leukocyte trafficking , inflammation , genetics , antibody , chemokine
Leukocyte trafficking from blood into tissue is a fundamental process in immune surveillance and the immune response to stimuli. Experimental autoimmune uveitis (EAU) is an animal model for posterior uveitis and is mediated by T lymphocytes and macrophages that infiltrate the posterior segment of the eye. To analyze leukocyte migration into retinal tissue during the course of EAU, labeled cells were identified in vivo by scanning laser ophthalmoscopy and in retinal flat‐mounts by confocal microscopy. Adhesion of blood leukocytes to retinal endothelial cells in vivo was significantly raised 48 h before the appearance of clinical disease, and this correlated with the increased expression of CD54 on retinal vessels. Mitogen‐activated spleen cells and CD4 + T cells only entered into retinal tissue in animals with clinical disease and not naive recipients. The disease status of the donor animal had no effect on leukocyte trafficking. These results, which identify leukocyte‐endothelial cell interactions in vivo , suggest that the activation of the retinal endothelium is a prerequisite to leukocyte adhesion and extravasation into ocular tissue during EAU. J. Leukoc. Biol . 64: 434–440; 1998.