Premium
Adoptive immunotherapy of cancer using monocyte‐derived macrophages: rationale, current status, and perspectives
Author(s) -
Andreesen Reinhard,
Hennemann Burkhard,
Krause Stefan W.
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.64.4.419
Subject(s) - adoptive cell transfer , immunotherapy , cancer research , ex vivo , cancer , cytotoxic t cell , immune system , immunology , cancer immunotherapy , in vivo , biology , cancer cell , medicine , in vitro , t cell , biochemistry , microbiology and biotechnology
Adoptive transfer of host defense cells may be able to correct an otherwise defective generation of competent immune cells in patients with cancer. Ex vivo ‐grown cytotoxic macrophages (MAC) able to recognize and destroy tumor cells but not normal cells are effective in murine models of metastasizing tumors. After the development of large‐scale technology to generate MAC in vitro from blood monocytes (MO), clinical trials in cancer patients have proven the feasibility and safety of infusing >3 × 10 9 autologous MO‐derived MAC activated by interferon‐γ or lipopolysaccharide. Various modalities of adoptive immunotherapy with human MAC have been realized: routes of application used were intravenous, intraperitoneal, intrapleural, and through selective hepatic artery perfusion. In addition, MAC have been generated from MO collected after granulyte‐macrophage colony‐stimulating factor treatment in vivo. Biodistribution studies using 111 indium‐labeled cells have revealed localization of MAC to sites of bulk tumor growth on regional infusion as well as to liver metastases on systemic application. Malignant ascites disappeared in about 50% of patients after intraperitoneal treatment, yet no other evidence of therapeutic efficacy of MAC could be demonstrated. Further advances of adoptive transfer of MO‐derived cells are developed with emphasis on the generation of antigen‐presenting cells primed in vitro with tumor cells or specific peptides. J. Leukoc. Biol . 64: 419–426; 1998.