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HIV load in highly purified CD8 + T cells retrieved from pulmonary and blood compartments
Author(s) -
Semenzato Gianpietro,
Agostini Carlo,
ChiecoBianchi Luigi,
De Rossi Anita
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.64.3.298
Subject(s) - biology , cd8 , bronchoalveolar lavage , immunology , immune system , lung , viral load , ctl* , cytotoxic t cell , t cell , asymptomatic , peripheral blood mononuclear cell , peripheral blood , virus , virology , pathology , medicine , biochemistry , in vitro
The pulmonary microenvironment can be HIV infected from the asymptomatic period of HIV infection and it has been demonstrated that HIV presence elicits a discrete virus‐specific CTL immune response in the lungs. In this study we analyzed T cell subsets and compared the proportion of CD8 + T cells harboring HIV in the lung and in the circulation of HIV‐seropositive patients with T cell alveolitis. Proviral load was assessed using the DNA‐polymerase chain reaction technique on highly purified CD8 + T cells isolated from peripheral blood and bronchoalveolar lavage. The proviral load of pulmonary CD8 + T cells showed an upward trend with respect to corresponding samples isolated from the peripheral blood of the same patients. J. Leukoc. Biol . 64: 298–301; 1998.