Premium
Expression of mRNA encoding neurotrophins and neurotrophin receptors in human granulocytes and bone marrow cells–enhanced neurotrophin‐4 expression induced by LTB 4
Author(s) -
Laurenzi Maria Assunta,
Beccari Tommaso,
Stenke Leif,
Sjolinder Mikael,
Stinchi Sofia,
Lindgren Jan Ake
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.64.2.228
Subject(s) - biology , neurotrophin , microbiology and biotechnology , receptor , neurotrophin 3 , messenger rna , bone marrow , granulocyte , immunology , neurotrophic factors , gene , genetics , brain derived neurotrophic factor
Abstract The expression of neurotrophin and neurotrophin receptor mRNAs in human granulocytes and bone marrow cells was examined using ribonuclease protection assay and reverse transcription‐polymerase chain reaction. The granulocytes expressed mRNA coding for nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF), and neurotrophin‐4 (NT‐4), but not neurotrophin‐3 (NT‐3). Moreover, the inflammatory mediator leukotriene B 4 (LTB 4 ) up‐regulated the expression of NT‐4 mRNA in granulocytes, but did not affect the expression of other neurotrophin mRNAs. Granulocytes generally lacked expression of mRNA coding for neurotrophin receptors. In contrast, human bone marrow cells consistently expressed mRNA for trkB (the BDNF and NT‐4 receptor) and displayed variable expression of mRNA coding for trkA (the tyrosine kinase NGF receptor) and LNGFR (the low‐affinity NGF receptor), whereas mRNA for trkC (the NT‐3 receptor) was not expressed. Contrary to granulocytes, normal bone marrow cells generally expressed only low levels of mRNA encoding BDNF and NT‐4. Expression of mRNA encoding NGF and NT‐3 was not detected. However, significantly increased expression of BDNF mRNA was observed when bone marrow cells from patients with chronic myeloproliferative disorders (MPD) were analyzed. The results suggest that neurotrophins may act as granulocyte‐derived effector molecules and that human bone marrow cells may be targets for these compounds, in particular BDNF and NT‐4. J. Leukoc. Biol . 64: 228–234; 1998.