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Profile of human macrophage transcripts: insights into macrophage biology and identification of novel chemokines
Author(s) -
Chantry David,
DeMaggio Anthony J.,
Brammer Heather,
Raport Carol J.,
Wood Christi L.,
Schweickart Vicki L.,
Epp Angela,
Smith Aaron,
Stine Johnny T.,
Walton Kim,
Tjoelker Larry,
Godiska Ronald,
Gray Patrick W.
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.64.1.49
Subject(s) - biology , macrophage , chemokine , identification (biology) , macrophage inflammatory protein , computational biology , microbiology and biotechnology , immunology , immune system , ecology , genetics , in vitro
High throughput partial sequencing of randomly selected cDNA clones has proven to be a powerful tool for examining the relative abundance of mRNAs and for the identification of novel gene products. Because of the important role played by macrophages in immune and inflammatory responses, we sequenced over 3000 randomly selected cDNA clones from a human macrophage library. These sequences represent a molecular inventory of mRNAs from macrophages and provide a catalog of highly expressed transcripts. Two of the most abundant clones encode recently identified CC chemokines. Macrophage‐derived chemokine (MDC) plays a complex role in immunoregulation and is a potent chemoattractant for dendritic cells, T cells, and natural killer cells. The chemokine receptor CCR4 binds MDC with high affinity and also responds by calcium flux and chemotaxis. CCR4 has been shown to be expressed by Th2 type T cells. Recent studies also implicate MDC as a major component of the host defense against human immunodeficiency virus. J. Leukoc. Biol . 64: 49–54; 1998.