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CD28‐mediated activation in CD45RA + and CD45RO + T cells: enhanced levels of reactive oxygen intermediates and c‐Rel nuclear translocation in CD45RA + cells
Author(s) -
Lahdenpohja Nina,
Hurme Mikko
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.63.6.775
Subject(s) - chromosomal translocation , biology , reactive oxygen species , cd28 , microbiology and biotechnology , oxygen , t cell , immunology , biochemistry , gene , immune system , chemistry , organic chemistry
We have analyzed the effect of complete T cell activation (anti‐CD3 plus anti‐CD28) on the activation of NF‐κB in CD45RA + (naive) and CD45RO + (memory/effector) T cells. Long exposure (24 h) induced stronger NF‐κB DNA binding in CD45RA + cells than in CD45RO + cells. Analysis of the nuclear c‐Rel protein indicated that after anti‐CD3 + anti‐CD28 stimulation the level of c‐Rel was higher in CD45RA + cells. Analysis of the cytoplasmic inhibitor IκBα indicated that anti‐CD3 + anti‐CD28 stimulation induced a long‐lasting degradation in CD45RA + cells but in CD45RO + cells the degradation process was more rapid. Because the CD28 costimulus is known to induce the production of reactive oxygen intermediates (ROIs), the intracellular ROI levels in CD45RA + and CD45RO + cells were compared by flow cytometry. ROIs were produced in both cell types, but more strongly in CD45RA + cells. The data presented in this study further emphasize the differences between CD45RA + and CD45RO + T lymphocytes in ROI‐dependent signaling pathways. J. Leukoc. Biol . 63: 775–780; 1998.