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Localization of eosinophil‐derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes
Author(s) -
Sur Sanjiv,
Glitz Dohn G.,
Kita Hirohito,
Kujawa Stephen M.,
Peterson Ellen A.,
Weiler Deborah A.,
Kephart Gail M.,
Wagner Jill M.,
George Terry J.,
Gleich Gerald J.,
Leiferman Kristin M.
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.63.6.715
Subject(s) - eosinophil cationic protein , eosinophil , major basic protein , biology , microbiology and biotechnology , buffy coat , eosinophil peroxidase , granulocyte , immunology , asthma
Eosinophil‐derived neurotoxin (EDN) and eosinophil cationic protein (ECP) are generally regarded as eosinophil‐specific proteins. We tested whether EDN and ECP are present in mature neutrophils. By indirect immunofluorescence, both eosinophils and neutrophils stained with antibodies to EDN and ECP. Lysates of purified (<0.1% eosinophil contamination) neutrophils contained EDN, 112 ± 4 ng/10 6 cells, and ECP, 163 ± 2 ng/10 6 cells, whereas eosinophil major basic protein (MBP) was not detectable. Electron microscopic examination of immunogold‐labeled buffy coat cells stained with EDN antibody showed that EDN is localized to neutrophil granules. Finally, EDN mRNA was detected in lysates of highly purified neutrophils (0.001% eosinophil contamination) by the reverse transcription‐polymerase chain reaction. We conclude that proteins that are either identical to or immunologically cross‐reactive with EDN and ECP are present in neutrophils and that EDN is synthesized and localized to neutrophil granules. Thus, caution must be exercised in interpreting the presence of EDN and ECP as specific markers of eosinophil‐associated inflammation in human disease. J. Leukoc. Biol . 63: 715–722; 1998.