Premium
Modulation of neutrophil adherence to endothelial cells by platelet‐derived adherence‐inhibiting factor through interactions with selectin molecules
Author(s) -
Iwabuchi Kazuhisa,
Nagaoka Isao,
Yamashita Tatsuhisa
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.63.4.500
Subject(s) - thrombin , tumor necrosis factor alpha , affinity chromatography , umbilical vein , platelet , p selectin , microbiology and biotechnology , monoclonal antibody , biology , immunology , biochemistry , in vitro , antibody , platelet activation , enzyme
Platelet‐derived adherence‐inhibiting factor (AIF) has been demonstrated to regulate the neutrophil binding to type IV collagen. In this study, we have examined the effect of AIF on neutrophil adherence to confluently cultured human umbilical vein endothelial cells (EC). AIF inhibited neutrophil adherence to thrombin‐ or tumor necrosis factor α (TNF‐α)‐stimulated EC by 75 or 43%, respectively, but hardly affected neutrophil adherence to resting EC. It is interesting to note that the inhibitory activity of AIF was reduced by N ‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) stimulation of neutrophils. Pretreatment of neutrophils or EC with AIF inhibited neutrophil adherence to thrombin‐ or TNF‐α‐stimulated EC, suggesting that neutrophils and EC have AIF‐binding proteins. Using AIF‐Sepharose affinity chromatography, AIF‐binding proteins containing L‐selectin were isolated from 125 I‐labeled resting neutrophils. However, L‐selectin was markedly decreased in the AIF‐binding fraction from fMLP‐stimulated neutrophils. With the use of AIF‐affinity chromatography, P‐ and E‐selectins were obtained in the AIF‐binding fractions from resting, thrombin‐, and TNF‐α‐stimulated EC. It is important to note that P‐ and E‐selectin were greatly increased in the AIF‐binding fractions from thrombin‐ and TNF‐α‐stimulated EC, respectively. Furthermore, AIF was able to bind to L‐selectin‐IgG chimeric protein and inhibit the binding of chimeric protein to thrombin‐ or TNF‐α‐stimulated EC. In addition, AIF inhibited the binding of anti‐P‐ or anti‐E‐selectin monoclonal antibody to the lysates of thrombin‐ or TNF‐α‐stimulated EC. Together these observations indicate that AIF could recognize L‐, P‐, and E‐selectins, and modulate neutrophil adherence to EC through interactions with selectin molecules. J. Leukoc. Biol . 63: 500–508; 1998.