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Chemokine synthesis in the HSV‐1‐infected cornea and its suppression by interleukin‐10
Author(s) -
Tumpey Terrence M.,
Cheng Hao,
Yan XiaoTian,
Oakes John E.,
Lausch Robert N.
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.63.4.486
Subject(s) - biology , cornea , hsl and hsv , chemokine , immunology , interleukin 4 , virology , cytokine , immune system , virus , neuroscience
Herpes simplex virus type 1 (HSV‐1) infection of the murine cornea results in a tissue‐destructive inflammatory response. In this study we show that virus infection induces the synthesis of macrophage inflammatory protein‐2 (MIP‐2), MIP‐1α, and monocyte chemoattractant protein‐1 (MCP‐1). However, only the production of MIP‐2 and MIP‐1α coincided with the influx of leukocytes into the cornea. IL‐10 treatment markedly suppressed chemokine message and protein synthesis in vivo. Local administration of IL‐10 also dramatically reduced the number of T cells and neutrophils migrating into the cornea and suppressed the severity of corneal disease. The inflammatory response could also be suppressed by the passive transfer of neutralizing antibody to MIP‐1α but not MCP‐1. We conclude that local IL‐10 administration can suppress chemokine synthesis, thereby ameliorating corneal disease. Furthermore, our results indicate that MIP‐1α plays a major role in herpes stromal keratitis development, whereas MCP‐1 does not. J. Leukoc. Biol . 63: 486–492; 1998.