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Transduction of recombinant human erythropoietin receptor cDNA into daughter progenitors derived from single CD34 3+ cord blood cells changes the differentiation profile of daughter progenitors
Author(s) -
Lu Li,
Li ZhiHua,
He Jie,
Ge Yue,
Rice Susan,
Broxmeyer Hal E.
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.63.3.389
Subject(s) - erythropoietin receptor , biology , complementary dna , progenitor cell , microbiology and biotechnology , erythropoietin , erythropoiesis , megakaryocyte , cell culture , stem cell , signal transduction , genetics , gene , medicine , anemia
In this study, we tested the capacity to change the differentiation profile of progenitor cells by retroviral‐mediated transduction of EpoR cDNA into one of the paired daughter cells derived from single CD34 3+ CB cells. Our results show that for the non‐viral‐treated daughter cells, the majority (99.6%) formed the same colony type. However, with cells transduced with viral vectors, 7.1% of the daughter cells transduced with the EpoR cDNA formed either a burst forming unit‐erythroid (BFU‐E) or a colony‐forming unit‐granulocyte, macrophage, erythroid, megakaryocyte (CFU‐GEMM) colony compared to the other daughter cell transduced with viral supernatant lacking EpoR cDNA, which formed either a colony‐forming unit granulocyte‐macrophage (CFU‐GM) or a high proliferative potential‐colony forming cell (HPP‐CFC) colony. Expression of the transduced EpoR cDNA was confirmed in individual colonies by RT‐PCR analysis. These results substantiate in a more rigorous fashion our previous results that it is possible to change the Epo‐responsive differentiation profile of progenitor cells by transduction into these cells of an EpoR cDNA and this change was apparent only in daughter cells derived from single CD34 3+ kit + cells transduced with EpoR cDNA. J. Leukoc. Biol . 63: 389–394; 1998.