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Subcellular localization of glycosphingolipids in human neutrophils
Author(s) -
Kniep Bernhard,
Skubitz Keith M.
Publication year - 1998
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.63.1.83
Subject(s) - lactosylceramide , biology , glycosphingolipid , subcellular localization , biochemistry , glycolipid , membrane , microbiology and biotechnology , staining , cell , globotriaosylceramide , cytoplasm , pathology , fabry disease , medicine , genetics , disease
The subcellular distribution of five major glycosphingolipids (GSLs) in human neutrophils was analyzed. The neutrophils were isolated from the blood of six donors and subdivided in three fractions containing the cell membranes, and the primary and the secondary granules, respectively. The separation was confirmed with antibodies detecting established subcellular fraction‐specific molecules. The two main neutral GSLs Galβ 1‐4Glcβ 1‐1 'Cer (lactosylceramide, LacCer) and nLc 4 Cer (paragloboside, PG) and the three gangliosides IV 3 NeuAcnLc 4 Cer (2‐3SPG), IV 6 NeuAcnLc 4 Cer (2‐6SPG), and VI 3 NeuAcnLc 6 Cer (2‐3SnHC) were quantitated using the immunochemical digoxigenin (DIG) staining procedure. Secondary granules contained the highest amount of these GSLs. They are followed by the primary granules and the cell membranes. Based on this quantitation, we conclude that the majority of the GSLs of neutrophils occur intracellularly. These findings are in striking contrast to the general assumption of GSLs being mainly concentrated in the cell membrane. J. Leukoc. Biol . 63: 83–88; 1998.