Premium
Ultraviolet irradiation accelerates apoptosis in human polymorphonuclear leukocytes: protection by LPS and GM‐CSF
Author(s) -
Sweeney John F.,
Nguyen Phu Kim,
Omann Geneva M.,
Hinshaw Daniel B.
Publication year - 1997
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.62.4.517
Subject(s) - apoptosis , lipopolysaccharide , biology , granulocyte , immunology , in vitro , neutrophile , programmed cell death , phagocytosis , microbiology and biotechnology , inflammation , cancer research , biochemistry
Polymorphonuclear leukocytes (PMN) play a central role in host response to injury and infection. Understanding factors that regulate PMN survival may therefore have a major influence on the development of novel treatment strategies for controlling life‐threatening infections, as well as local and systemic inflammatory responses. Unfortunately, the presently utilized in vitro culture model of PMN apoptosis makes the examination of early biochemical events surrounding PMN apoptosis very difficult. This study demonstrates that a short course of UV irradiation (15 min) can be used to induce rapid progression of PMN through the apoptotic process with 70–90% of PMN displaying features of apoptosis by 4 h after UV exposure. Bacterial lipopolysaccharide and granulocyte‐macrophage colony‐stimulating factor, which are known to prolong PMN survival during in vitro culture, also protected PMN from UV‐accelerated apoptosis. The UV‐accelerated model of PMN apoptosis provides another valuable tool for the investigation of early signaling pathways associated with inducing or delaying PMN apoptosis. J. Leukoc. Biol. 62: 517–523; 1997.