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Modulation of nitric oxide production in RAW 264.7 cells by transforming growth factor‐beta and interleukin‐10: differential effects on free and encapsulated immunomodulator
Author(s) -
Seyler Isabelle,
Appel Martine,
Devissaguet JeanPhilippe,
Legrand Philippe,
Barratt Gillian
Publication year - 1997
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.62.3.374
Subject(s) - muramyl dipeptide , nitric oxide , transforming growth factor beta , transforming growth factor , interleukin , biology , cell culture , beta (programming language) , biochemistry , cytokine , endocrinology , immunology , in vitro , genetics , computer science , programming language
Abstract The mouse macrophage cell line RAW 264.7 can be stimulated to produce nitric oxide (NO) by muramyltripeptide cholesterol included within biodegradable poly(D,L‐lactide) nanocapsules (NC MTP‐Chol). The aim of this work was to determine whether one or both of the cytokines transforming growth factor‐beta (TGF‐β) and interleukin‐10 (IL‐10) could be responsible for feedback control seen at high concentrations. Activated RAW 264.7 cells produced TGF‐β1. When exogenous TGF‐β1 was added during stimulation, a dose‐dependent inhibition of NO production was observed when NC MTP‐Chol was used, whereas activation by the soluble muramyl dipeptide (MDP) was not affected. Furthermore, addition of a blocking antibody to TGF‐β arrested the fall in NO production seen at high concentrations of NC MTP‐Chol. Addition of IL‐10 during RAW 264.7 cell activation also reduced NO production; however, in this case, both NC MTP‐Chol and MDP were equally affected. The presence of anti‐IL‐10 antibody during activation significantly increased NO production. J. Leukoc. Biol. 62: 374–380; 1997.