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Contrasting effects of α‐ and β‐androstenediol on oncogenic myeloid cell lines in vitro
Author(s) -
Huynh P. N.,
Loria R. M.
Publication year - 1997
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.62.2.258
Subject(s) - biology , apoptosis , cell culture , in vitro , myelopoiesis , myeloid , cell growth , microbiology and biotechnology , tunel assay , programmed cell death , thymidine , cancer research , progenitor cell , biochemistry , genetics , stem cell
Abstract The in vitro effects of 17αAED, the isomer of 5‐androstene‐3β,17β diol (17βAED) on the basal growth of murine RAW 264.7, P388D1, and human HL‐60 cells were investigated. 17αAED treatment of RAW cells for 48 h reduced total cell number without increasing cell death as detected by trypan blue exclusion. At these doses, DNA synthesis as measured by [ 3 H]thymidine incorporation was suppressed by as much as 65%, P < 0.05. This effect was time‐ and dose‐dependent and reversible on removal of the steroid. Similar results were obtained with P388D1 and human HL‐60 cell lines. At 50 nM or above, 17αAED induced apoptosis in RAW cells and HL‐60 as detected by transmission electron microscopy and TUNEL assays. By contrast, treating cells with the isomer 17βAED had no such effect. These data suggest that the balance between the anti‐proliferative effect of 17αAED and the proliferative effects of 17βAED may determine the overall level of myelopoiesis. J. Leukoc. Biol. 62: 258‐267; 1997.