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Leukotriene B 4 ‐activated human endothelial cells promote transendothelial neutrophil migration
Author(s) -
Nohgawa Masaharu,
Sasada Masataka,
Maeda Akinori,
Asagoe Kohsuke,
Harakawa Nari,
Takano Kuniko,
Yamamoto Kokichi,
Okuma Minoru
Publication year - 1997
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.62.2.203
Subject(s) - leukotriene b4 , chemotaxis , extravasation , umbilical vein , human umbilical vein endothelial cell , n formylmethionine leucyl phenylalanine , endothelial stem cell , biology , neutrophile , immunology , granulocyte , in vitro , microbiology and biotechnology , receptor , inflammation , biochemistry
We explored the effect of leukotriene B 4 (LTB 4 ) on endothelial cells in LTB 4 ‐induced transendothelial migration (TEM) of neutrophils as an in vitro model of neutrophil extravasation. Chemotactic response of human neutrophils to LTB 4 was significantly lower than that in response to N ‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP), whereas the extent of TEM in response to LTB 4 was significantly higher than that to fMLP. The study on random migration induced by LTB 4 and fMLP also showed similar results, which indicated that LTB 4 might affect the human umbilical cord vein endothelial cell (HUVEC) barrier. Neutrophil TEM was induced by pretreatment of HUVEC monolayer with LTB 4 but not with fMLP. Treatment of endothelial cells by ONO‐4057, a LTB 4 receptor antagonist, abolished the effect of LTB4 almost completely whereas neutrophils treated with ONO‐4057 could transmigrate through HUVEC treated with LTB 4 . These findings indicated that LTB 4 could induce neutrophil TEM by acting on HUVEC. J. Leukoc. Biol. 62: 203–209; 1997.