Leukotriene B 4 ‐activated human endothelial cells promote transendothelial neutrophil migration | Zendy

Nohgawa Masaharu | Zendy; Sasada Masataka | Zendy; Maeda Akinori | Zendy; Asagoe Kohsuke | Zendy; Harakawa Nari | Zendy; Takano Kuniko | Zendy; Yamamoto Kokichi | Zendy; Okuma Minoru | Zendy

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Leukotriene B 4 ‐activated human endothelial cells promote transendothelial neutrophil migration

Author(s) -

Nohgawa Masaharu,

Sasada Masataka,

Maeda Akinori,

Asagoe Kohsuke,

Harakawa Nari,

Takano Kuniko,

Yamamoto Kokichi,

Okuma Minoru

Publication year - 1997

Publication title -

journal of leukocyte biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.819

H-Index - 191

eISSN - 1938-3673

pISSN - 0741-5400

DOI - 10.1002/jlb.62.2.203

Subject(s) - leukotriene b4 , chemotaxis , extravasation , umbilical vein , human umbilical vein endothelial cell , n formylmethionine leucyl phenylalanine , endothelial stem cell , biology , neutrophile , immunology , granulocyte , in vitro , microbiology and biotechnology , receptor , inflammation , biochemistry

We explored the effect of leukotriene B 4 (LTB 4 ) on endothelial cells in LTB 4 ‐induced transendothelial migration (TEM) of neutrophils as an in vitro model of neutrophil extravasation. Chemotactic response of human neutrophils to LTB 4 was significantly lower than that in response to N ‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP), whereas the extent of TEM in response to LTB 4 was significantly higher than that to fMLP. The study on random migration induced by LTB 4 and fMLP also showed similar results, which indicated that LTB 4 might affect the human umbilical cord vein endothelial cell (HUVEC) barrier. Neutrophil TEM was induced by pretreatment of HUVEC monolayer with LTB 4 but not with fMLP. Treatment of endothelial cells by ONO‐4057, a LTB 4 receptor antagonist, abolished the effect of LTB4 almost completely whereas neutrophils treated with ONO‐4057 could transmigrate through HUVEC treated with LTB 4 . These findings indicated that LTB 4 could induce neutrophil TEM by acting on HUVEC. J. Leukoc. Biol. 62: 203–209; 1997.

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