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Porcine bone marrow myeloid cells: phenotype and adhesion molecule expression
Author(s) -
Summerfield Artur,
McCullough Kenneth C.
Publication year - 1997
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.62.2.176
Subject(s) - biology , myeloid , haematopoiesis , cd14 , microbiology and biotechnology , progenitor cell , myelopoiesis , bone marrow , cluster of differentiation , cell adhesion molecule , cd44 , immunology , cell , stem cell , flow cytometry , genetics
The purpose of this study was the discrimination of porcine bone marrow hematopoietic cell (BMHC) subpopulations and cell maturation stages by using cluster of differentiation (CD) and Swine Workshop Cluster (SWC) molecules, combined with cell size, granularity, and the capacity to generate an oxidative burst. SWC3 was the earliest myeloid marker identified in the differentiation pathways. Through SWC3/SWC8 double labeling, three populations committed to the myeloid lineage were discriminated: early myeloid progenitors, which were SWC3 low SWC8 – ; cells committed to the granulocytic lineage, which were SWC3 + SWC8 + ; monocytic cells, which were SWC3 + ‐SWC8 – . The SWC3 low SWC8 – myeloid progenitors expressed high levels of CD44 and CD49d, but no CD14. Among the SWC3 + SWC8 + granulocytic cells, the most immature were CD14 – CD11a/18 – CD44 low , an intermediate stage was CD14 + CD11a/18 low CD44 – , and the most mature were CD14 + CD11a/18 high –CD44 high . Only the latter could generate a rapid oxidative burst. Moreover, during granulocytic maturation, a constant decrease in CD49d/29 expression was observed. In contrast, the SWC3 + SWC8 – monocytic cells were mainly CD14 + and expressed high levels of the adhesion molecules. These results define porcine hematopoietic differentiation and maturation stages. Similarities in human and porcine adhesion molecule expression have been identified, suggesting common functionalities in the regulation of hematopoiesis and the potential of the pig as a model for hematopoietic studies. J. Leukoc. Biol. 62: 176–185; 1997.