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Monocytes from mobilized stem cells inhibit T cell function
Author(s) -
Ino Kazuhiko,
Singh Rakesh K.,
Talmadge James E.
Publication year - 1997
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.61.5.583
Subject(s) - biology , cd14 , percoll , microbiology and biotechnology , cytotoxic t cell , cd8 , t cell , interleukin 3 , progenitor cell , interleukin 21 , stem cell , immunology , immune system , flow cytometry , biochemistry , in vitro
Granulocyte‐macrophage colony‐stimulating factor, mobilized peripheral blood stem cell (PSC) products, and peripheral blood leukocytes posttransplantation contain cells that cause allogeneic and autologous T cell apoptosis. Isolation and characterization of these cells demonstrated that they were low‐density (Percoll fractionation) CD14 + monocytes. T cells in PSC products have a depressed phytohemagglutinin (PHA) mitogenic response; however, purified CD4 + or CD8 + T cells exhibit a statistically normal mitogenic function. Furthermore, no T cell inhibitory activity was observed in CD14 + , CD4 + , and CD8 + cell‐depleted fractions enriched in CD4 – CD8 – TCRα/β + T cells. Inhibition of T cell function by CD14 + monocytes required cell‐cell contact, and the analyses of DNA fragmentation by Southern and TUNEL analysis demonstrates an activation‐induced T cell apoptosis in the presence of CD14 + monocytes. Reverse‐transcriptase polymerase chain reaction studies suggested that high levels of interleukin‐10 or tumor necrosis factor gene transcripts in the PSC products may contribute to the inhibition of T cell function. J. Leukoc. Biol. 61: 583–591; 1997.