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Macrophage differentiation and expression of macrophage colony‐stimulating factor in murine milky spots and omentum after macrophage elimination
Author(s) -
Zhu Hong,
Naito Makoto,
Umezu Hajime,
Moriyama Hiroshi,
Takatsuka Hisakazu,
Takahashi Kiyoshi,
Shultz Leonard D.
Publication year - 1997
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.61.4.436
Subject(s) - biology , macrophage , macrophage colony stimulating factor , immunology , microbiology and biotechnology , genetics , in vitro
To elucidate the differentiation mechanisms of macrophages in the murine omentum, we studied the repopulation of these cells and the expression of macrophage colony‐stimulating factor (M‐CSF) in the milky spots and omental tissues in mice depleted of macrophages following administration of liposome‐encapsulated dichloromethylene diphosphonate (clodronate). The macrophages in the omentum were spindle or dendritic in shape, expressed several macrophage‐specific antigens and Ia antigen, and phagocytized intraperitoneally injected carbon particles. In the milky spots, macrophages and macrophage precursors were detected, and the number of precursors increased alter elimination of macrophages by intraperitoneal injection of liposome‐encapsulated clodronate. Macrophage precursors in the milky spots proliferated, moved to the omentum, and transformed into dendritic‐shaped macrophages. Expression of M‐CSF mRNA extracted from the milky spots was markedly enhanced at 2 and 3 days after macrophage depletion. Localization of M‐CSF protein and mRNA was observed in the stromal cells of the milky spots. In osteopetrosis ( op/op ) mutant mice that are defective in the production of functional M‐CSF omental macrophages were absent. These results indicate that M‐CSF locally produced in the milky spots plays an important role in providing a microenvironment for development and differentiation of omental macrophages. J. Leukoc. Biol . 61: 436–444; 1997.