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Dynamic imaging of neutrophil migration in three dimensions: mechanical interactions between cells and matrix
Author(s) -
Mandeville John T. H.,
Lawson Moira A.,
Maxfield Frederick R.
Publication year - 1997
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.61.2.188
Subject(s) - biology , matrix (chemical analysis) , microbiology and biotechnology , extracellular matrix , composite material , materials science
Fluorescence confocal microscopy was used to obtain three‐dimensional (3‐D) images of human neutrophils migrating through a 3‐D matrix of amniotic membrane with a temporal resolution of 30–60 s and a spatial resolution of 2 μm in the z‐dimension. Neutrophils migrating in response to a chemoattractant gradient within a 3‐D matrix were apparently able to generate traction by use of lateral pseudopods inserted into footholds in the matrix as evidenced by matrix distortion. Similar anchored pseudopods were seen in cells migrating across polycarbonate membranes with 0.8‐μm pores; the presence of these pores increased cell polarization and migration compared with cells on membranes without pores. Expansion of pseudopods distal to narrow constrictions in the matrix and porous filters was observed and appeared to be used to pull cells through the openings. Neutrophils deformed parts of the elastic amnion matrix during migration without permanently altering the substrate. Contact guidance of neutrophils crawling along matrix fibrils was also observed. These observations show that neutrophils migrating in 3‐D are able to utilize mechanical structures in the matrix, not present on 2‐D surfaces, to generate traction for locomotion. J. Leukoc. Biol . 61: 188–200; 1997.