Premium
Enhancement of immune response to naked DNA vaccine by immunization with transfected dendritic cells
Author(s) -
Manickan Eianchezhiyan,
Kanangat Sivadasan,
Rouse Richard J. D.,
Yu Zhiya,
Rouse Barry T.
Publication year - 1997
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.61.2.125
Subject(s) - biology , dna vaccination , immunization , naked dna , transfection , immune system , virology , dendritic cell , immunology , gene gun , dna , gene , genetics
Immunization with plasmid DNA encoding various proteins promises to be a valuable vaccine approach especially if its immunogenicity could be optimized. In this study we show that the intramuscular delivery in dendritic cells (DC) of naked plasmid DNA encoding two proteins of herpes simplex virus (HSV) leads to the induction of significantly enhanced levels of resistance to viral challenge. Whereas DC transfected in vitro with DNA induced enhanced immunity, similarly transfected macrophage (M ɸ ) populations lacked immunogenicity even though plasmid expression occurred in vitro. The enhanced immunity induced by DC‐delivered DNA appeared to be associated mainly with an increased Thl CD4 + T cell response. Our results add evidence that DC are the essential antigen‐presenting cell types involved in immune responses to intramuscularly administered DNA vaccines. J. Leukoc. Biol . 61: 125–132; 1997.