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Interleukin‐4 rapidly down‐modulates the macrophage colony‐stimulating factor receptor in murine macrophages
Author(s) -
Sbarba Persio Dello,
Rovida Elisabetta,
Caciagli Barbara,
Nencioni Lucia,
Labardi Danilo,
Paccagnini Alessandro,
Savini Luca,
Cipolleschi Maria Grazia
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.60.5.644
Subject(s) - biology , macrophage , macrophage colony stimulating factor , microbiology and biotechnology , receptor , activator (genetics) , colony stimulating factor , interleukin 4 , cytokine , immunology , biochemistry , in vitro , stem cell , haematopoiesis
The activation of macrophages interferes with their response to macrophage colony‐stimulating factor (M‐CSF), the main growth and differentiation factor for mononuclear phagocytes. We tested the rapid effects of interleukin‐4 (IL‐4), the alternative macrophage activator produced by Th2 helper lymphocytes, on the receptor for M‐CSF (M‐CSFR) expressed on the cell surface of murine macrophages. IL‐4 rapidly down‐modulated M‐CSFR in a dose‐dependent fashion. This effect was unique to IL‐4 among a number of Th2‐produced cytokines, none of which, with the exception of IL‐4 itself, is able to activate macrophages. The down‐modulation of M‐CSFR by IL‐4 was partially prevented by the inhibition of the activity of phospholipase C or protein kinase C. The data are consistent with the hypothesis that the down‐modulation of M‐CSFR is a property common to, and exclusive of, macrophage activators, and is driven by different activators via a common mechanism. J. Leukoc. Biol . 60: 644–650; 1996.