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Increased plasma GlyCAM‐1, a mouse L‐selectin ligand, in response to an inflammatory stimulus
Author(s) -
Suguri Takayasu,
Kikuta Akio,
Iwagaki Hiromi,
Yoshino Tadashi,
Tanaka Noriaki,
Orita Kunzo
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.60.5.593
Subject(s) - inflammation , stimulation , cell adhesion molecule , biology , extracellular , selectin , immunology , cell adhesion , microbiology and biotechnology , endocrinology , biochemistry , cell
GlyCAM‐1 (glycosylation‐dependent cell adhesion molecule‐1) is one of the sialomucin‐like ligands for L‐selectin, which is a member of the selectin family and mediates initial adhesion of leukocytes to specialized high endothelial venules in lymph nodes and venules at sites of inflammation. GlyCAM‐1, lacking a transmembrane domain, is supposed to be secreted into the blood, To understand the functional role of secreted GlyCAM‐1, we performed sandwich enzyme‐linked immunosorbent assay to measure GlyGAM‐1 plasma levels after inflammatory stimulus. BALB/c mice were injected with complete Freund's adjuvant (CFA) in the hind footpads; serum levels of GlyCAM‐1 and L‐selectin bound to GlyCAM‐1 and several inflammatory cytokines, including interleukin‐6 (IL‐6), were measured at various intervals. IL‐6 showed a significant increase 3 h after CFA stimulation. GlyGAM‐1 was increased at 3 h, reached peak levels at 12 h, and gradually decreased thereafter. Levels of L‐selectin bound to the plasma GlyCAM‐1 changed over a similar time course, reached peak at 12 h after, and then began to decrease. The binding of L‐selectin to plasma GlyCAM‐1 was completely eliminated with the presence of ethyleneglycol‐bis(β‐aminoethylether)‐ N , N '‐tetraacetic acid, showing the calcium dependency of this binding. These findings show that GlyGAM‐1 release is enhanced by inflammatory stimulation and also suggest that released plasma GlyGAM‐1 may trap, at least in part, soluble L‐selectin shed from stimulated leukocytes to neutralize each other. J. Leukoc. Biol . 60: 593–597; 1996.