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Gadolinium chloride inhibits Kupffer cell nitric oxide synthase (iNOS) induction
Author(s) -
Roland Claude R.,
Naziruddin Bashoo,
Mohanakumar T.,
Flye M. Wayne
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.60.4.487
Subject(s) - nitric oxide , phagocytosis , kupffer cell , nitric oxide synthase , western blot , lipopolysaccharide , biology , in vivo , biochemistry , microbiology and biotechnology , immunology , endocrinology , gene
Kupffer cells (KC) are the phagocytic macrophages of the liver. The rare earth metal, gadolinium (GdCl 3 ), is a lanthanide, which, after phagocytosis by the KC, has been found to alter various aspects of KC physiology. In this study, we describe for the first time that the in vivo administration of GdCl 3 to rate decreases the release of NO by isolated rat KC in response to lipopolysaccharide. Western blot analysis shows decreased expression of both imlucible nitric oxide synthase as well as total cellular calmodulin after GdCl 3 treatment. Possible mechanisms for this phenomenon are suggested. J. Leukoc. Biol 60: 487–492; 1996.