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Anti‐CD69 antibodies enhance phorbol‐dependent glucose metabolism and Ca 2+ levels in human thymocytes. Antagonist effect of cyclosporin A
Author(s) -
Conde M.,
Montaño R.,
MorenoAurioles V. R.,
Ramirez R.,
SanchezMateos P.,
SanchezMadrid F.,
Sobrino F.
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.60.2.278
Subject(s) - diacylglycerol kinase , biology , glycolysis , thymocyte , pyruvate kinase , phosphofructokinase , hexokinase , monoclonal antibody , biochemistry , protein kinase c , antigen , microbiology and biotechnology , endocrinology , signal transduction , metabolism , cd8 , antibody , immunology
The human activation antigen CD69 is an early inducible surface glycoprotein acquired by T cells in the thymus at the stage of positive selection and during activation of mature lymphoid cells both in vivo and in vitro. We have studied the regulatory influence of CD69 activation pathway on the glycolytic process and transduction signals of thymocytes. Treatment of human thymocytes with different anti‐CD69 monoclonal antibodies (mAbs), in the presence of submitogenic doses of phorbol ester, produced an enhanced release of lactate without significant alterations in Fru 2,6‐P 2 levels or phosphofructokinase‐2 (PFK‐2) and pyruvate kinase activities. A small increase in phosphofructokinase‐1 (PFK‐1) activity was also detected. Furthemore, anti‐CD69 mAb increased the glucose detritiation from [2‐ 3 H] and [3‐ 3 H]glucose, thus indicating an enhanced flux through hexokinase and PFK‐1 steps. In addition, de novo synthesis of diacylglycerol and intracellular Ca 2+ levels increased after anti‐CD69 mAb treatment. The stimulatory effects of anti‐CD69 mAb on both glycolysis and Ca 2+ levels were inhibited by cyclosporin A. Because CD69 molecules are present in certain subset populations of immature thymocytes, the ability of anti‐CD69 mAb to stimulate the glycolysis, the synthesis of diacylglycerol and the intracellular Ca 2+ levels suggest that the activation signals delivered through CD69 molecules could play a role in the thymus cells maturation. J. Leukoc. Biol 60: 278–284; 1996.