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Fibronectin enhances the migration rate of human neutrophils in vitro
Author(s) -
Everitt Elizabeth A.,
Malik Asrar B.,
Hendey Bill
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.60.2.199
Subject(s) - vitronectin , fibronectin , chemotaxis , integrin , extracellular matrix , microbiology and biotechnology , biology , in vitro , monoclonal antibody , receptor , fibronectins , immunology , biochemistry , antibody
Efficient polymorphonuclear neutrophil (PMN) migration depends on specific interactions between PMNs, endothelial cells, and extracellular matrix (ECM) proteins. We investigated the relationship between PMN migration and the ECM molecule fibronectin (FN). We used an in vitro migration assay system to show that human PMNs migrated across an FN‐coated filter barrier toward a formyl‐Met‐Leu‐Phe (fMLP) chemoattractant gradient in greater numbers than across (uncoated) bare filters. In 1 h of fMLP stimulation, 69 ± 6% of the PMNs had migrated across the FN‐coated filters, whereas 46 ± 5% of PMNs migrated across bare filters. This effect was specific to FN; coating the filters with the ECM protein vitronectin did not enhance migration. Monoclonal antibodies against FN or against the α 5 or β 1 integrin subunits of the FN receptor inhibited the enhanced PMN migration response across FN‐coated filters. These findings indicate that the extracellular matrix protein FN enhances PMN migration and that this response is mediated by the α 5 β 1 FN receptor. J. Leukoc. Biol . 60: 199–206; 1996.