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Effects of spaceflight on rat peripheral blood leukocytes and bone marrow progenitor cells
Author(s) -
Ichiki A. T.,
Gibson L. A.,
Jago T. L.,
Strickland K. M.,
Johnson D. L.,
Lange R. D.,
Allebban Z.
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.60.1.37
Subject(s) - neutrophilia , bone marrow , erythropoietin , biology , progenitor cell , spaceflight , immunology , spleen , granulocyte , monocytosis , white blood cell , haematopoiesis , myelopoiesis , peripheral blood cell , blood cell , peripheral blood mononuclear cell , andrology , stem cell , endocrinology , medicine , microbiology and biotechnology , biochemistry , aerospace engineering , engineering , in vitro
The white blood cell (WBC) elements and the bone marrow myeloid progenitor cell populations were analyzed to ascertain adaptation to microgravity and subsequent readaptation to 1 G in rats flown on the 14‐day Spacelab Life Sciences‐2 (SLS‐2) mission. Bone marrow cells were harvested from one group of rats killed inflight (FD13) and blood was drawn from three other groups at various times. The WBC level was normal on FD14 with the exception of neutrophilia. On FD13, numbers of colony‐forming units‐granulocyte (CFU‐G), CFU‐GM, and CFU‐M from flight animals were decreased compared with ground controls when incubated with recombinant rat interleukin‐3 (rrIL‐3) alone or in combination with recombinant human erythropoietin (rhEpo). On recovery (R + 0), flight rats had decreased numbers of total leukocytes and absolute numbers of lymphocytes and monocytes with elevated neutrophils compared with control rats. They had lower numbers of CD4, CD8, CD2, CD3, and B cells in the peripheral blood but no differences in spleen lymphocytes.