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Dissecting the human immune system with single cell RNA sequencing technology
Author(s) -
Xu Gang,
Liu Yang,
Li Hanjie,
Liu Lei,
Zhang Shuye,
Zhang Zheng
Publication year - 2020
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.5mr1019-179r
Subject(s) - biology , computational biology , immune system , transcriptome , rna , workflow , single cell sequencing , cell , single cell analysis , rna seq , immunology , gene , genetics , computer science , gene expression , phenotype , exome sequencing , database
Single‐cell RNA sequencing (scRNA‐seq) is a powerful new technology allowing the analysis of transcriptomes from individual cell and is ideally suited to dissect immune cell heterogeneity. ScRNA‐seq has already been applied to identify novel immune cell subsets, elaborate cellular differentiation trajectories, and elucidate immunopathogenic mechanisms. Here, we briefly discuss the recent progresses and challenges in the scRNA‐seq technology including the workflow, recent applications in immunology, and potential hurdles that need to be overcome. This review will highlight how single cell technology promotes our understanding of human immunology.