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Killing of phagocytosed Staphylococcus aureus by human neutrophils requires intracellular free calcium
Author(s) -
Wilsson åsa,
Lundqvist Helen,
Gustafsson Mikael,
Stendahl Olle
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.59.6.902
Subject(s) - calcium , extracellular , phagocytosis , staphylococcus aureus , biology , calcium in biology , intracellular , microbiology and biotechnology , neutrophil extracellular traps , opsonin , bacteria , biochemistry , immunology , chemistry , inflammation , genetics , organic chemistry
Abstract The mobilization of intracellular calcium plays an important role in regulating neutrophil activation. With this in mind we investigated the effect of intra‐ and extracellular calcium on the ability of human neutrophils to kill complement‐opsonized Staphylococcus aureus. We found that a rise in intracellular calcium is necessary for efficient killing of phagocytosed S. aureus. In the presence of extracellular calcium, killing of ingested bacteria in calcium‐buffered neutrophils compared with normal cells was slightly reduced. Calcium buffering had no effect on phagocytic uptake by the neutrophils, but did decrease the generation of toxic oxygen metabolites, measured as chemiluminescence (CL). In non‐depleted and calcium‐depleted cells, removal of extracellular calcium did not affect ingestion but did cause a marked decrease in the ability to kill the bacteria. In parallel, the CL response was substantially reduced or completely blocked. These data show that calcium is not a prerequisite for phagocytosis of S. aureus by human neutrophils, but does play a vital role in the post‐ingestion killing of the bacteria by regulating the generation of toxic oxygen metabolites.