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Paradoxical effects of colchicine on the activation of human neutrophils by chemotactic factors and inflammatory microcrystals
Author(s) -
Roberge C. J.,
Gaudry M.,
Gilbert C.,
Malawista S. E.,
Médicis R.,
Lussier A.,
Poubelle P. E.,
Naccache P. H.
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.59.6.864
Subject(s) - colchicine , tyrosine phosphorylation , phosphorylation , superoxide , nocodazole , biology , tyrosine , chemotaxis , microbiology and biotechnology , protein tyrosine phosphatase , biochemistry , cytoskeleton , genetics , receptor , cell , enzyme
Neutrophil activation by chemotactic factors and by inflammatory microcrystals is accompanied by increases in protein tyrosine phosphorylation and by the activation of the NADPH oxidase. The addition of colchicine inhibited both responses induced by triclinic monosodium urate or calcium pyrophosphate crystals. On the other hand, colchicine enhanced the tyrosine phosphorylation of specific protein in neutrophils stimulated by chemotactic factor and augmented the production of superoxide anions induced by these same agonists. The effects of colchicine were shared by other anti‐microtubule agents (nocodazole and vinblastine) but not by its inactive analogue β‐lumicolchicine, trimethylcolchicinic acid, indomethacin, or phenyl‐butazone. Furthermore, the (enhancing as well as inhibitory) effects of colchicine on tyrosine phosphorylation and superoxide anion production were reversed by taxol. Finally, in human cytoplasts colchicine again inhibited microcrystal‐stimulated tyrosine phosphorylation but did not change chemotactic factor‐stimulated phosphorylation. These data strongly support the hypothesis that microtubule‐related mechanisms are involved in the modulation of the tyrosine phosphorylation response in human neutrophils, and suggest that a relationship may exist between the augmentation of tyrosine phosphorylation and of the stimulation of the NADPH oxidase induced by chemotactic factors.