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Augmentations of glucose uptake and glucose transporter‐1 in macrophages following thermal injury and sepsis in mice
Author(s) -
Gamelli Richard L,
Liu Hong,
He LiKe,
Hofmann Cecilia A.
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.59.5.639
Subject(s) - glut1 , lipopolysaccharide , tumor necrosis factor alpha , glucose transporter , biology , macrophage , sepsis , endocrinology , medicine , carbohydrate metabolism , deoxyglucose , inflammation , messenger rna , microbiology and biotechnology , in vitro , immunology , biochemistry , insulin , gene
Glucose is the primary metabolic substrate of macrophages, which are critical components of the host response to injury and infection. We have carried out a series of studies to examine macrophage glucose uptake and the status of glucose transporter 1 (GLUTI) at both the mRNA and protein level. Peritoneal macrophages that were obtained from mice undergoing sham burned (S), 15%TBSA burn (B)± Pseudomonas aeruginosa burn infection (B + I) and lipopolysaccharide (LPS) or tumor necrosis factor‐α (TNF‐α) administration. [ 3 H]deoxyglucose uptake was significantly increased (B, 157 ± 9%; B + I, 243 ± 19%; S + LPS, 231 ± 24%; S + TNF‐α, 379 ± 18%; B + LPS, 230 ± 13%; and B + TNF, 305 ± 23%, P < 0.01 vs. sham). GLUTI mRNA and protein levels were increased as well (mRNA: B, 135 ± 13%; B + I, 250 ± 33%; S + LPS, 282 ± 29%; S + TNF‐α, 193 ± 19%; B + LPS, 378 ± 20%; and B + TNF‐α, 204 ± 16%; protein: B, 159 ± 27%; B + I, 181 ± 17%; S + LPS, 219 ± 26%; S + TNF‐α, 343 ± 51%; B + LPS, 366 ± 41%; and B + TNF‐α, 415 ± 44, P < 0.01 vs. sham). Macrophages co‐cultured with LPS or TNF‐α in vitro demonstrated a similar response pattern. Following burn injury and infection, macrophages augment their cellular glucose uptake, which is facilitated by an increased GLUT1 mRNA and protein levels. TNF‐α elicited by LPS may mediate this enhanced carbohydrate metabolism at the point of glucose entry into the cell.