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Rapid secretion of intracellularly pre‐stored interleukin‐8 from rabbit platelets upon activation
Author(s) -
Su ShaoBo,
Mukaida Naofumi,
Matsushima Kouji
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.59.3.420
Subject(s) - biology , secretion , platelet , rabbit (cipher) , microbiology and biotechnology , interleukin , immunology , cytokine , endocrinology , statistics , mathematics
Abstract Freshly isolated rabbit platelets contained a substantial amount of intracellular interleukin‐8 (IL‐8) as revealed by immunohistochemistry and Western blotting analysis. Thrombin stimulation at room temperature induced platelets to secrete IL‐8 rapidly, and the maximal secretion was observed within 10 min after the activation. Secreted IL‐8 possessed neutrophil chemotactic activity that was neutralised by a specific neutralizing monoclonal antibody to rabbit IL‐8 but not a control one. These results suggest that rabbit platelets store biologically active IL‐8 intracellularly and secrete it rapidly upon activation. This study implies that platelets may be a pivotal participant in inflammatory reactions in rabbits through producing cytokines and chemokines, including IL‐8.