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Silicotic lymph node reactions in mice: genetic differences, correlation with macrophage markers, and independence from T lymphocytes
Author(s) -
Weirich Uta,
Friemann Johannes,
Rehn Bernd,
Henkelüdecke Uwe,
Lammers Thorsten,
Sorg Clemens,
Bruch Joachim,
Gleichmann Ernst
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.59.2.178
Subject(s) - lymph node , biology , macrophage , strain (injury) , antigen , hyperplasia , lymph , lymphatic system , histopathology , microbiology and biotechnology , immunology , pathology , endocrinology , medicine , anatomy , in vitro , genetics
Quartz was injected into a hind foot of BALB/c and DBA/2 mice and on days 40, 90, and 180 the progressive response ensuing in the draining popliteal lymph node (PLN) was investigated by histopathology and immunohistopathology. The area of silicotic nodules (ASN) was measured by morphometry, and, by this parameter, strain BALB/c proved to be a high responder to quartz, and strain DBA/2 a low responder, albeit both strains showed a similar degree of reactive lymphoid hyperplasia in the draining PLN. Both strains also showed a similar quartz content in the draining PLN but in BALB/c mice quartz particles were concentrated in the ASN, whereas in DBA/2 mice they were evenly dispersed over the PLN. Because the silicotic response of athymic BALB/c nu/nu mice was even stronger than that of euthymic BALB/c mice, T cells are not required for the development of silicotic nodules. This fits the notion that quartz is not an antigen and that high and low responder strains are MHC‐identical. Because quartz‐treated BALB/c, but not DBA/2 mice, showed a persistent expression of the macrophage differentiation markers MRP8 and MRP14, phenotypically the observed strain difference in silicotic responsiveness seems to be expressed at the level of macrophages.