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Lipopolysaccharide regulates cysteine‐rich intestinal protein, a zinc‐finger protein, in immune cells and plasma
Author(s) -
Hallquist Nora A.,
Khoo Christina,
Cousins Robert J.
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.59.2.172
Subject(s) - immune system , biology , lipopolysaccharide , peripheral blood mononuclear cell , spleen , messenger rna , immunocytochemistry , acute phase protein , microbiology and biotechnology , immunology , endocrinology , inflammation , biochemistry , gene , in vitro
Abstract Cysteine‐rich intestinal protein (CRIP), a double zinc‐finger LIM protein, is expressed in great abundance in the intestine. We have found comparable levels of CRIP mRNA in peritoneal macrophages, peripheral blood mononuclear cells (PBMC), and lesser amounts in thymus and spleen. Because CRIP expression was high in immune cells, rats were challenged with lipopolysaccharide (LPS) to determine whether expression was altered during the acute‐phase immune response. Immunocytochemistry showed that, in adherent mononuclear cells, CRIP protein was localized in the cytoplasm. CRIP mRNA levels increased over time after LPS injection in peritoneal macrophages, PBMC, spleen, and intestine. No changes in CRIP mRNA level were seen in either liver or thymus. In PBMC, the level of CRIP mRNA decreased before increasing later in the acute‐phase immune response. CRIP protein was found in the plasma. Shortly after LPS administration plasma CRIP decreased, suggesting that CRIP was either passively diffused out of capillaries or was actively shunted into tissues to execute its function. Increased CRIP expression seen in response to IPS suggests that CRIP may play a role in immune cell activation or differentiation or in processes associated with cellular repair.