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Role of eosinophil‐chemotactic C‐C chemokines in cutaneous inflammation
Author(s) -
Schröder JensM.,
Noso Norio,
Sticherling Michael,
Christophers Enno
Publication year - 1996
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.59.1.51
Subject(s) - eosinophil , chemotaxis , chemokine , atopic dermatitis , immunology , biology , western blot , microbiology and biotechnology , inflammation , heparin , biochemistry , receptor , gene , asthma
In the dermal sites of atopic skin, eosinophil (Eo) granule protein or more rarely intact Eos represent a characteristic histological feature. We addressed the question of whether lesional scales of patients with various eosinophilic skin disorders contain Eo attractant and tried to characterize it biochemically. In scales of a patient with drug reaction, heparin‐binding Eo attractants could be identified. High‐performance liquid chromatographic analyses together with specific ELISA and Western blot analyses revealed identity with RANTES. No other heparin‐binding Eo chemotaxin could be identified. HPLC analysis of pooled lesional scale extracts of patients with atopic dermatitis showed fractions containing only weak heparin‐binding Eo‐chemotactic activity, which, however, showed RANTES immunoreactivity. In experiments to elucidate the putative cellular origin of Eo‐attracting chemokines in human skin we investigated supernatants of atopic skin–derived T lymphocytes as well as supernatants of stimulated dermal fibroblasts for Eo‐chemotactic factors. Unexpectedly, we did not find any heparin‐bound Eo attractants in supernatants of stimulated cultured atopic skin–derived T lymphocyte clones, whereas fibroblasts produced RANTES as well as granulocyte‐macrophage colony‐stimulating factor. Therefore, fibroblasts are a likely source of eosinophil attractant cells, which could contribute to the Eo infiltrate. Selectivity of the infiltrate might come from selective induction of RANTES and/or induction of other as yet unidentified Eo‐specific chemokines.