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Relationship between light scattering in flow cytometry and changes in shape, volume, and actin polymerization in human polymorphonuclear leukocytes
Author(s) -
Keller H.U.,
Fedier A.,
Rohner R.
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.58.5.519
Subject(s) - polymerization , flow cytometry , shape change , biology , measure (data warehouse) , biophysics , volume (thermodynamics) , actin , granulocyte , materials science , optics , immunology , microbiology and biotechnology , physics , composite material , polymer , thermodynamics , computer science , database
Using two different cytometers, an Epics Profile II and a FACScan, we determined the extent to which changes in forward and right angle scatter are a reliable measure for changes in polymorphonuclear leukocyte (PMN) shape, volume, and actin polymerization and whether distinct types of shape changes in PMNs can be recognized. PMN stimulation can substantially change the positions of PMNs in the scatter diagram of the FACScan but not of the Epics Profile II. Within the limits of the experiments, it has been possible to determine whether or not a shape change has taken place using the FACScan but not using the Epics Profile II. However, using either cytometer, it has not been possible to determine which type of shape change (e.g., spherical vs. polarized vs. nonpolar cells) has taken place. Furthermore, forward or right angle scatter changes are not a reliable measure for changes in cell volume or actin polymerization of human PMNs.