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Histamine enhances granulocyte‐macrophage colony‐stimulating factor and interleukin‐6 production by human peripheral blood mononuclear cells
Author(s) -
Mor Shlomith,
Nagler Ar,
Barak Vivian,
Handzel Zeev T.,
GellerBernstein Carmi,
Fabian Ina
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.58.4.445
Subject(s) - peripheral blood mononuclear cell , biology , histamine , granulocyte macrophage colony stimulating factor , immunology , granulocyte , peripheral blood , macrophage , interleukin , cytokine , endocrinology , in vitro , biochemistry
The effect of histamine on the production of cytokines by subpopulations of mononuclear cells was studied. A 3.5‐fold increase in the number of myeloid colony‐forming units (CFU‐C) was observed when bone marrow cells were cultured in the presence of conditioned medium prepared from nonadherent mononuclear cells cultured with 10 −4 M histamine (CM‐histamine) compared with phosphate‐buffered saline (CM‐PBS). Using ELISA and radioimmunoassay kits, histamine was found to enhance the production of GM‐CSF (9.6‐fold) and IL‐6 (8.2‐fold) by mononuclear cells but not by nonadherent cells or large granular lymphocytes. Anti‐GM‐CSF and anti‐IL‐6 antibodies markedly blocked cytokine activity in CM‐PBS, whereas the blocking effect in CM‐histamine was moderate, indicating enhanced GM‐CSF and IL‐6 activity in CM‐histamine. No GM‐CSF or IL‐6 levels could be detected in CM‐histamine or CM‐PBS prepared from CD3 + , CD4 + , or CD8 + lymphocytes. Preincubation of CM‐histamine with H 1 and H 2 receptor antagonists resulted in complete blocking of the histamine‐enhanced colony‐stimulating activity. We conclude that histamine is able to activate human mononuclear cells to generate cytokines such as GM‐CSF and IL‐6 via H 1 and H 2 receptors.