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Recombinant human IL‐7 administration in mice affects colony‐forming units‐spleen and lymphoid precursor cell localization and accelerates engraftment of bone marrow transplants
Author(s) -
Boerman Otto C.,
Gregorio Theresa A.,
Grzegorzewski Krzysztof J.,
Faltynek Connie R.,
Kenny James J.,
Wiltrout Robert H.,
Komschlies Kristin L.
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.58.2.151
Subject(s) - biology , spleen , bone marrow , recombinant dna , immunology , lymphatic system , cancer research , gene , genetics
Murine reconstitution assays were used to investigate the effects of recombinant human interleukin‐7 (rhIL‐7) on myeloid and lymphoid precursors and on bone marrow engraftment. Reconstitution with bone marrow from rhIL‐7‐treated mice results in a 3.4‐fold decrease in total colony‐forming unit‐spleen (CFU‐S) activity (day 9) and an 18.1‐ and 11.9‐fold decrease in its ability to generate thymocytes and splenic B lineage cells, respectively. In contrast, after reconstitution with splenocytes from rhIL‐7‐treated mice, CFU‐S activity increased 23.6‐fold (day 9) and the thymocyte and splenic B lineage cell regenerative capacity increased by 4.0‐ and 3.2‐fold, respectively. In addition, CD43 low+ , B220 low+ cells that contain pre‐pro‐B cells and pro‐B cells were expanded two‐ to threefold and Igμ ‐ , B220 + , CD2 ‐ and Igμ ‐ , B220 + , CD2 + B lineage cells were expanded approximately 10‐fold and 10‐ to 45‐fold (depending on the tissue examined), respectively, after rhIL‐7 treatment Administration of rhIL‐7 to irradiated mice transplanted with bone marrow resulted in accelerated T cell and B cell reconstitution by up to 2–4 weeks. Thus, rhIL‐7 administration affects the distribution of myeloid and lymphoid precursors. Moreover, rhIL‐7 administration accelerates murine bone marrow cell engraftment and therefore may be useful in reducing the engraftment time in bone marrow transplant patients. J. Leukoc. Biol. 58: 151–158; 1995.