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Elevation of rainbow trout Oncorhynchus mykiss macrophage respiratory burst activity with macrophage‐derived supernatants
Author(s) -
Jang Seon I.,
Hardie Laura J.,
Secombes Christopher J.
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.6.943
Subject(s) - biology , rainbow trout , respiratory burst , macrophage , tumor necrosis factor alpha , lipopolysaccharide , monoclonal antibody , trout , macrophage activating factor , microbiology and biotechnology , antibody , immunology , medicine , endocrinology , biochemistry , in vitro , fishery , fish <actinopterygii>
Abstract A variety of supernatants were prepared by stimulating rainbow trout Oncorhynchus mykiss head kidney macrophages with lipopolysaccharide (LPS), tumor necrosis factor α (TNF‐α), or a leucocyte‐derived macrophage‐activating factor (I‐MAF), individually and in combination. If generated using a 12‐h stimulation period, such supernatants were found to elevate significantly the respiratory burst activity of target macrophages; that is, they contained a macrophage‐derived MAF (m‐MAF), but supernatants generated using a shorter incubation period showed no significant activity. Combinations of these treatments were particularly effective in generating m‐MAF–containing supernatants. The elevation of respiratory burst activity by supernatants generated using combined treatments could be partially inhibited by prior treatment of the target macrophages with anti‐TNF‐α receptor 1 (TNFR1) monoclonal antibodies (mAbs). Similarly, treatment of macrophages with combinations of 1‐MAF and m‐MAF generated supernatants with potent m‐MAF activity and this activity was partially inhibited by prior treatment of the target cells with anti‐TNFR1 mAb. In addition, the presence of anti‐transforming growth factor β 1 (TGF‐β 1 ) serum while generating these latter supernatants resulted in significantly increased m‐MAF activity. Such data suggest that fish leukocytes secrete a variety of potent macrophage‐activating (TNF‐α) and ‐deactivating (TGF‐β) factors. J . Leukoc . Biol . 57: 943–947; 1995.