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Macrophage colony stimulatory factor–activated bone marrow macrophages suppress lymphocytic responses through phagocytosis: a tentative in vitro model of Rosai‐Dorfman disease
Author(s) -
Jadus Martin R.,
Sekhon Sant,
Barton Beverly E.,
Wepsic H. Terry
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.6.936
Subject(s) - phagocytosis , biology , macrophage , in vitro , bone marrow , immunology , cancer research , microbiology and biotechnology , biochemistry
NBXFO hybridoma cells produced macrophage colony‐stimulating factor (M‐CSF), which stimulated the growth of murine bone marrow–derived macrophages with potent suppressor activity. These macrophages suppressed lymphocyte responses to mitogens and antigens in a dose‐dependent manner. Using a transwell chamber, we demonstrated that macrophages needed physical contact with the lymphocytes to suppress lymphocyte proliferation on day 1 in the concanavalin A mitogen reaction. In addition, no soluble suppressor factor was detected at that time. The number of lymphocytes disappeared with time when they were cocultured with the macrophages. Electron microscopy revealed that the macrophages phagocytosized the lymphocytes after 7 1/2 h. Dextran sulfate, heparan, and fucoidan prevented the macrophages from suppressing the lymphocytes. This phenomenon resembles the human disease sinus histiocytosis, also called Rosai‐Dorfman disease, in which macrophages (histiocytes) phagocytosize autologous lymphocytes; occasionally, this disease is associated with immunological abnormalities. Thus we believe that macrophage‐activating cytokines, such as M‐CSF, may stimulate macrophages to phagocytose lymphocytes in vivo. J . Leukoc. Biol. 57: 936–942; 1995.