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Interferon‐α and interferon‐γ down‐regulate the production of interleukin‐8 and ENA‐78 in human monocytes
Author(s) -
SchnyderCandrian Silvia,
Strieter Robert M.,
Kunkel Steven L.,
Walz Alfred
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.6.929
Subject(s) - chemotaxis , recombinant dna , biology , interferon , lipopolysaccharide , interleukin , interferon gamma , cytokine , northern blot , monocyte , immunology , western blot , interleukin 8 , microbiology and biotechnology , messenger rna , gene , biochemistry , receptor
The two chemotactic cytokines interleukin‐8 (IL‐8) and epithelial neutrophil activating protein 78 (ENA‐78) were recently shown to be potent chemoattractants and activators of neutrophil function and to be present in certain inflammatory diseases. We have studied the effects of recombinant and natural interferon‐α (IFN‐α) and of recombinant interferon gamma (rIFN‐γ) on the production of EL‐8 and ENA‐78 in lipopolysaccharide‐ and interleukin‐1–stimulated human monocytes. Both types of interferons showed a strong, concentration‐dependent inhibition of neutrophil‐stimulating bioactivity. Similarly, the secretion of IL‐8 and ENA‐78 was also inhibited by up to 73%. Northern blot experiments demonstrated that IFN‐α decreases the steady‐state levels of IL‐8 and ENA‐78 mRNA in monocytes, suggesting that IFN‐α as well as IFN‐γ may control the expression of neutrophil chemotactic cytokines at the mRNA level. J . Leukoc . Biol. 57: 929–935; 1995.