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Cell line cross‐contamination of U‐397
Author(s) -
Reid Yvonne A.,
O'Neill Kathleen,
Chen T.R.,
Dorotinsky Catherine,
McGuire Linda,
Macy Marv,
McCuntock Patrick,
Hay Robert
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.5.804
Subject(s) - biology , security token , cell culture , karyotype , stock (firearms) , contamination , botany , microbiology and biotechnology , genetics , chromosome , ecology , gene , computer science , mechanical engineering , computer security , engineering
Abstract To the Editor: The human histiocytic lymphoma cell line, U‐937, established by S. Sundström and K. Nilsson in 1974 (1), was deposited at American Type Culture Collection (ATCC) in 1981 by H. Koren, Duke University Medical Center, Durham, N.C. A starter culture obtained from the depositor was propagated through one passage to generate a “token” freeze (F‐2704). Progenies from ampules of this cryopreserved material were expanded to produce the “seed” stock (F‐2717) and initial distribution stocks. The seed stock is retained to generate new distribution stocks that are provided to scientific investigators. Cultures derived from token, seed, and distribution stocks were tested for microbial and mycoplasma contamination. The cell line species of origin was confirmed by routine isoenzymology and karyology.