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Immune complexes increase nitric oxide production by interferon‐γ‐stimulated murine macrophage‐like J774.16 cells
Author(s) -
Mozaffarian Neelufar,
Berman Joan W.,
Casadevall Arturo
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.4.657
Subject(s) - isotype , biology , immune system , nitric oxide , nitrite , macrophage , interferon gamma , monoclonal antibody , antibody , antigen , immune complex , cytokine , immunology , microbiology and biotechnology , biochemistry , in vitro , endocrinology , ecology , nitrate
Murine macrophage‐like J774.16 cells were tested for changes in nitric oxide production upon incu bation with immune complexes. Cryptococcus neofor mans capsular polysaccharide and polysaccharidespecific monoclonal antibodies were added to J774.16 cells in the presence and absence of recombinant murine interferon‐γ (IFN ‐ γ) . The effect of immune complexes on nitrite synthesis was both concentration dependent and isotype dependent. In the presence of IFN‐γ, immune complexes of IgGl, IgG2a, IgG2b, or IgG3 isotype in creased nitrite levels, whereas complexes of IgM isotype did not. Immune complexes did not alter nitrite production by unstimulated macrophages. Antibody alone, anti gen alone, and antigen with irrelevant IgGl antibody did not augment nitrite formation, either in the presence or absence of IFN‐γ. indicating a requirement for FCγR cross‐linking. These results suggest that IgG isotypes may offer additional protection against pathogens by enhancing macrophage nitric oxide production , J. Lcukoc. Biol . 57: 657–662; 1995.