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Effects of granulocyte colony‐stimulating factor and interferon‐γ on antifungal activity of human polymorphonuclear neutrophils against pseudohyphae of different medically important Candida species
Author(s) -
Roilides Emmanuel,
Holmes Andrew,
Blake Cassann,
Pizzo Philip A.,
Walsh Thomas J.
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.4.651
Subject(s) - hypha , microbiology and biotechnology , candida albicans , biology , candida parapsilosis , granulocyte , candida tropicalis , interferon gamma , corpus albicans , neutrophile , in vitro , immunology , cytokine , biochemistry
Abstract Polymorphonuclear neutrophils (PMNs) are the major host defense against pseudohyphae, the inva sive form of Candida species. We studied the effects of granulocyte colony‐stimulating factor (G‐CSF) and interfcron‐γ (IFN‐γ) on the PMN‐induced damage of pseudohyphae of Candida albicans, Candida tropicalis , and Candida parapsilosis in vitro by using two antifungal assays: a modified limiting dilution assay and a colorimet ric metabolic 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tctraznlium bromide assay. PMNs from healthy volunteers were ineubated with either G‐CSF (100‐10,000 U/ml) or IFN‐γ (10‐5000 U/ml) or buffer at 37° C for 90 min and their capacity to damage nonopsonized pseudohyphae was then measured. C. tropicalis appeared to be the most susceptible species, whereas C. parapsilosis showed the highest rate of rcsistancc to PMN damage. G‐CSF (500–10 T 000 U/ml) and IFN‐γ (100‐1000 U/ml) enhanced the antifungal activity of PMNs against C. albicans pseudo hyphae (P < .01 and P < .05). Among the others, G‐CSF enhanced PMN‐induccd damage of C. parapsilosis at concentrations 500–10,000 U/ml (P < .05), whereas it enhanced damage of C, tropicalis only at 10,000 U/ml (P < .01), IFN‐γ (100–1000 U/ml) primed PMNs also caused augmented damage of C. parapsilosis (P < .05) but not of C, tropicalis at the same concentrations. Species‐dependent differences exist in the responses of PMNs to Candida pseudohyphae and G‐CSF as well as IFN‐γ are important immunomodulators of phagocytic host defenses against them Leukoc. Biol . 57: 651–656; 1995.