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T cells and HIV‐induced T cell syncytia exhibit the same motility cycle
Author(s) -
Sylwester Andrew,
Shutt Damon,
Wessels Deborah,
Stapleton Jack T.,
Stites Jan,
Kennedy Ronald C.,
Soil David R.
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.4.643
Subject(s) - motility , biology , syncytium , peripheral blood mononuclear cell , microbiology and biotechnology , cell cycle , cell culture , cell , in vitro , biochemistry , genetics
Ameboid cells ranging in complexity from Dictyostelsum amebas to human polymorphonuclear leu kocytes (PMNs) translocate in a cyclical fashion. Using computer‐assisted motion analysis, we have analyzed the motility of human lymphocytes of the immortal SupTl cell line and of a peripheral blood mononuclear cell popu lation highly enriched for CD4‐positive cells (CD4‐enriched PBMCs) on four substrates—plastic, dehydrated rat tail collagen, hydrated rat tail collagen, and bovine aortic en dothelium. In addition, we have analyzed the motility on these substrates of syncytia induced by human immuno deficiency virus (HIV) in cultures of both cell types. It is demonstrated that both SupTl cells and CD4‐enriched PBMCs exhibit a motility cycle with a period of 1.6 min that is independent of substrate, independent of average cell velocity, and similar to the periods of translocating Dictyostelium amebas and PMNs. More surprisingly, it is demonstrated that HIV‐induced SupTl and PBMG syn cytia with volumes 10 to 100 times those of single cells ex hibit the same motility cycle as their single‐cell progeni tors. These observations support the generality of the motility cycle in animal cells and, for the first time, demonstrate that the cycic is independent of cell size. J. Lcukoc. BioL 57; 643–650; 1995.