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Induction of macrophage binding to virally infected cells by low levels of lipopolysaccharide
Author(s) -
Derrick Steven C.,
LeBlanc Paul A.
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.4.569
Subject(s) - vesicular stomatitis virus , lipopolysaccharide , cytolysis , biology , 3t3 cells , macrophage , cell culture , microbiology and biotechnology , cytotoxicity , haematopoiesis , immunology , in vitro , biochemistry , transfection , stem cell , genetics
Bone marrow culture‐derived macrophages (BMCM) and vesicular stomatitis virus‐infected BALB/e‐3T3 cells (3T3‐VSV) were used to determine whether macrophages could be activated to bind virally infccted cells. Although lipopolysaccharide (LPS)‐activated BMCM bound some uninfected BALB/c‐3T3 cells, the number of targets that were bound increased with increasing times between infection and assay. Fur thermore, LPS‐activated BMCM bound more 3T3‐VSV cells than did control macrophages. As more targets were added, the number of targets bound by the unactivated macrophagcs remained relatively level. However, the number of targets bound by the activated macrophages increased with increasing concentrations of added targets until they reached a plateau that was eight times greater than that bound by the unactivated BMCM. When BMCM were exposed to LPS for 24 h before assay, they lost both their ability to bind to 3T3‐VSV and their cyto lytic activity against those targets. However, as when using P815, a standard tumor target, the acquisition of binding of 3T3‐VSV could be separated from macrophage cytolytic activity against those targets. The amount of LPS required to activated BMCM for increased binding of 3T3‐VSV cells was 10–100 times lower than that needed to induce cytolytic activity for 3T3‐VSV cells. Each of these values was approximately 100‐fold lower than the amount of LPS required to induce the corresponding ac tivity (binding or cytotoxicity) by using P815 targets . J. Lcukoc. Biol 57: 569–573; 1995.