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Retention of phagocytic functions in cryopreserved human monocytes
Author(s) -
Hansen JohnBjarne,
Halvorsen Dag Seeger,
Haldorsen Bjørg Christina,
Olsen Randi,
Sjursen Haakon,
Kierulf Peter
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.2.235
Subject(s) - phagocytosis , cryopreservation , internalization , flow cytometry , biology , microbiology and biotechnology , respiratory burst , peripheral blood mononuclear cell , staphylococcus aureus , immunology , ingestion , andrology , receptor , in vitro , bacteria , medicine , biochemistry , embryo , genetics
Phagocytosis and respiratory burst activity were measured by flow cytometry in fresh and cryopreserved human monocytes, after ingestion of Escherichia coli and Staphylococcus aureus . Mononuclear leukocytes, isolated from 15 healthy donors, were divided into two portions, of which one was examined immediately and the other was cryopreserved for 3 weeks. Morphological characteristics and expression of receptors involved in phagocytosis were similar in fresh and cryopreserved monocytes. Furthermore, both internalization of bacteria and respiratory burst activity remained unchanged after cryopreservation. Transmission electron microscopy confirmed actual internalization of bacteria and not merely bacterial attachment to monocytes. Monocytes were demonstrated to retain integral cellular functions during cryopreservation. This may imply that the method has potential for use in basal and clinical trials. J. Leukoc. Biol. 57: 235–241; 1995.