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Expression of both types of human interleukin‐8 receptors on mature neutrophils, monocytes, and natural killer cells
Author(s) -
Morohashi Hirohisa,
Miyawaki Toshio,
Nomura Hideki,
Kuno Kouji,
Murakami Seishi,
Matsushima Kouji,
Mukaida Naofumi
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.1.180
Subject(s) - biology , cd16 , receptor , immunology , microbiology and biotechnology , cd64 , polyclonal antibodies , antibody , cd3 , immune system , cd8 , biochemistry
cDNA cloning revealed the presence of two related but distinct types of human interleukin‐8 (IL‐8) receptors, type I (type A) and type II (type B). By immunizing rabbits with glutathione‐ S ‐transferase fused with the NH 2 ‐terminal domain of each type of IL‐8 receptor, we prepared polyclonal antibodies that specifically recognized the NH 2 ‐terminal domain of each type of IL‐8 receptor. Immunofluorescence analysis of human peripheral blood leukocytes demonstrated that mature granulocytes except eosinophils express both types of IL‐8 receptors. A majority of monocytes and CD16 + natural killer (NK) cells in peripheral blood were stained with both antibodies, whereas CD3 + T or CD20 + B lymphocytes in peripheral blood or CD34 + cells in cord blood were not stained with either antibody. These results suggest that both types of human IL‐8 receptors were coordinately and selectively expressed in mature granulocytes, monocytes, and CD16 + NK cells. J. Leukoc. Biol. 57: 180–187; 1995.