z-logo
Premium
Cellular target of in vitro–induced suppressor cells derived from the spleen of Mycobacterium lepraemurium–infected mice and role of IFN‐γ in their development
Author(s) -
Gosselin Diane,
Turcotte Raymond,
Lemieux Suzanne
Publication year - 1995
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.57.1.122
Subject(s) - biology , spleen , suppressor , in vitro , monoclonal antibody , interferon , interleukin 2 , microbiology and biotechnology , immunology , antibody , immune system , biochemistry , gene
Abstract Unfractionated spleen cells from C3H mice infected a few weeks before with Mycobacterium lepraemurium developed a suppressor activity after overnight culture. This requires contact of plastic adherent cells with nonadherent cells distinct from T, B, or natural killer cells. The present study demonstrates that anti‐interferon‐γ (IFN‐γ) monoclonal antibody and indomethacin totally abrogate the expression, although not the induction, of this activity. Furthermore, culture‐induced suppressor cells selectively inhibit T lymphocyte proliferation, probably by altering the generation of interleukin‐2 (IL‐2) responsiveness through reduction of the affinity and density of high‐affinity IL‐2 receptors on activated cells. These and other previously determined properties of culture‐induced suppressor cells, similar to those of adherent suppressor cells detected in freshly harvested spleen cells at a later stage of M. lepraemurium infection, suggest a common precursor. If so, the present observations should help in defining a strategy to prevent the impairment of cell‐mediated immunity in infected mice. J. Leukoc. Biol. 57: 122–128; 1995.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here