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Kinetics of macrophage subpopulations and expression of monocyte chemoattractant protein‐1 (MCP‐1) in bleomycin‐induced lung injury of rats studied by a novel monoclonal antibody against rat MCP‐1
Author(s) -
Sakanashi Yuji,
Takeya Motohiro,
Yoshimura Teizo,
Feng Lili,
Morioka Tohru,
Takahashi Kiyoshi
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.56.6.741
Subject(s) - monocyte , monoclonal antibody , immunohistochemistry , macrophage , northern blot , biology , microbiology and biotechnology , bleomycin , western blot , pulmonary alveolus , in situ hybridization , messenger rna , pathology , antibody , immunology , medicine , biochemistry , in vitro , gene , genetics , chemotherapy
We investigated the kinetics of macrophage subpopulations and the expression of monocyte chemoattractant protein 1 (MCP‐1) in a rat model of bleomycin‐induced lung injury. Rat macrophage subpopulations were examined by immunohistochemistry using various anti‐rat macrophage monoclonal antibodies (mAbs) and their proliferative capacity by [ 3 H]thymidine ( 3 HTdR) autoradiography. To detect the localization of expressed MCP‐1, we generated an mAb against rat MCP‐1 for immunohistochemical staining. Expression of MCP‐1 messenger RNA (mRNA) was detected by Northern blot hybridization. Shortly after intratracheal instillation of bleomycin, the number of exudate macrophages recognized by mAb TRPM‐3 increased in the injured lungs, peaked 3 days later, and decreased thereafter, whereas tissue macrophages identified by mAb ED2 increased slowly and peaked 2 weeks after instillation. Northern blot analysis disclosed that the expression of MCP‐1 mRNA in the lung was most prominent 1 day after instillation and declined thereafter, preceding the numerical change of the TRPM‐3‐positive exudate macrophages. Immunohistochemistry with anti‐rat MCP‐1 revealed that the main sources of MCP‐1 production were alveolar and interstitial macrophages and polymorphonuclear leukocytes. Based on these results, MCP‐1 produced by polymorphonuclear leukocytes and by alveolar and interstitial macrophages is thought to induce the infiltration of blood monocytes, and infiltrated exudate macrophages produce MCP‐1 to enhance subsequent accumulation of macrophages. In contrast, the expression of MCP‐1 did not correlate with the numerical changes of the ED2‐positive macrophages. J. Leukoc. Biol. 56: 741–750; 1994.