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Cellular composition of Corynebacterium pseudotuberculosis pyogranulomas in sheep
Author(s) -
Pépin Michel,
Pittet JeanChristophe,
Olivier Michel,
Gohin Isabelle
Publication year - 1994
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.56.5.666
Subject(s) - biology , corynebacterium pseudotuberculosis , macrophage , cd8 , lymph , immunohistochemistry , receptor , t cell , immunology , yersinia pseudotuberculosis , lymphocyte , t lymphocyte , microbiology and biotechnology , immune system , pathology , bacteria , gene , genetics , medicine , virulence , in vitro
The cellular composition of 46 pyogranulomas experimentally induced with Corynebacterium pseudotuberculosis in sheep was determined by immunohistochemistry. Lesions localized in inoculation sites or draining lymph nodes consisted of macrophage and lymphocyte layers distributed around a necrotic center and surrounded by a fibrous capsule. In immature lesions, T cells of the CD4 + subset predominated, whereas in mature lesions proportions of CD8 + T lymphocytes and cells expressing the γ / δ chains for the T cell receptor increased. Numerous pyogranuloma cells expressed the interleukin‐2 receptor. In addition to these general characteristics, a large individual variability in the proportions of macrophage and T cell subsets was observed for lesions of the same age, in particular for epithelioid macrophages. This heterogeneity suggests a different cellular pattern in relation to the persistence or the elimination of bacteria by the host. J. Leukoc. Biol . 56: 666–670; 1994.